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Key Documents

N6386

Sigma-Aldrich

Neomycin trisulfate salt hydrate

powder, suitable for cell culture, BioReagent

Synonyme(s) :

Antibiotic 10676, Neomycin B

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About This Item

Formule empirique (notation de Hill):
C23H46N6O13 · 3H2SO4 · xH2O
Numéro CAS:
Poids moléculaire :
908.88 (anhydrous basis)
Numéro CE :
Code UNSPSC :
12352207
ID de substance PubChem :
Nomenclature NACRES :
NA.76

product name

Neomycin trisulfate salt hydrate, powder, BioReagent, suitable for cell culture

Source biologique

Streptomyces sp.

Gamme de produits

BioReagent

Forme

powder

Puissance

≥600 μg neomycin per mg (Dried basis)

Conditionnement

pkg of 100 g
pkg of 25 g
pkg of 5 g

Technique(s)

cell culture | mammalian: suitable

Couleur

white to yellow

Solubilité

H2O: 50 mg/mL

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria

Mode d’action

protein synthesis | interferes

Température de stockage

2-8°C

Chaîne SMILES 

O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O

InChI

1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1

Clé InChI

WHAGUNPVKDUVFV-QGTTWHFQSA-N

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Description générale

As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days.
Chemical structure: aminoglycoside

Application

Neomycin trisulfate salt hydrate has been used:
  • to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants
  • for Escherichia coli antibiotic susceptibility test
  • in bone marrow transplantation of mice

Actions biochimiques/physiologiques

Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. Neomycin trisulfate functions as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.

Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.

Attention

Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.

Notes préparatoires

Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Inflammasome activation aggravates cutaneous Xanthomatosis and atherosclerosis in ACAT1 (acyl-CoA cholesterol acyltransferase 1) deficiency in bone marrow
Wakabayashi T, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 38(11), 2576-2589 (2018)
Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells
Yamahara K, et al.
Neuropharmacology, 119, 26-39 (2017)
Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (2014)
Anthony D Kappell et al.
Frontiers in microbiology, 6, 336-336 (2015-05-15)
Urban waterways represent a natural reservoir of antibiotic resistance which may provide a source of transferable genetic elements to human commensal bacteria and pathogens. The objective of this study was to evaluate antibiotic resistance of Escherichia coli isolated from the
Zhenhua Zhong et al.
Frontiers in cell and developmental biology, 8, 712-712 (2020-09-29)
Aminoglycoside-induced hair cell (HC) loss is one of the most important causes of hearing loss. After entering the inner ear, aminoglycosides induce the production of high levels of reactive oxygen species (ROS) that subsequently activate apoptosis in HCs. Citicoline, a

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Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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