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Key Documents

N4637

Sigma-Aldrich

Newborn Calf Serum

USA origin, sterile-filtered, suitable for cell culture

Synonyme(s) :

NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Source biologique

bovine (calf)

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Composition

hemoglobin, ≤20 mg/dL

Origine

USA origin

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤100 EU/mL endotoxin

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Application

Newborn calf serum (NCS) is collected within 10 days post partum. NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.

Notes préparatoires

Collected from calves that are 10 days old or less.

Remarque sur l'analyse

Endotoxin tested

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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Christopher C Nguyen et al.
Journal of virology, 94(5) (2019-12-06)
The human cytomegalovirus (HCMV) endoplasmic reticulum (ER)-resident glycoprotein UL148 is posited to play roles in immune evasion and regulation of viral cell tropism. UL148 prevents cell surface presentation of the immune cell costimulatory ligand CD58 while promoting maturation and virion
Kyoko Shimizu et al.
Cell biology international, 30(4), 381-388 (2006-03-15)
We have recently developed a primary culture system for visceral adipocytes (VAs) using stomal-vascular cells (SVCs) isolated from the mesenteric fat tissue of male Sprague-Dawley rats of 3-5 weeks of age. Modified Dulbecco's modified Eagle medium (DMEM)/F12 containing 17 microM
R Kishida et al.
Theriogenology, 62(9), 1663-1676 (2004-10-30)
To establish a defined in vitro maturation culture system for porcine oocytes, we examined the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation medium. Furthermore, to evaluate cytoplasmic maturation, we investigated GSH concentrations and embryo
Davide Camposampiero et al.
Cornea, 22(3), 254-258 (2003-03-27)
To explore the possibility of replacing bovine serum with chicken ovalbumin in cornea preservation. Twenty-one pairs of corneas were cultivated at 31 degrees C in a medium containing either 2% (vol/vol) of newborn calf serum (one cornea) or 2 mg/mL
Ling Chen et al.
American journal of nephrology, 25(5), 459-465 (2005-08-25)
Oxidative stress has been considered to be a common pathogenetic factor of diabetic nephropathy. But the reason why renal cells are susceptible to oxidative injury in diabetes is not clear. Vitamin C plays a central role in the antioxidant defense

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