N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Synonyme(s) : p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Formule linéaire : O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• Numéro CAS: 52483-84-8
• Poids moléculaire : 417.44
• Numéro Beilstein : 6553276
• Numéro CE : 257-949-0
• Numéro MDL: MFCD00013173
• Code UNSPSC : 12352204
• ID de substance PubChem : 24897623
• Nomenclature NACRES : NA.83

Propriétés

• product name: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Niveau de qualité: 300
• Pureté: ≥97% anhydrous basis
• Forme: powder
• Solubilité: water: 10 mg/mL, clear, colorless to faintly yellow
• Température de stockage: −20°C
• Chaîne SMILES: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• Clé InChI: KXNNUYCXODNFBD-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Actions biochimiques/physiologiques

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)