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MTOX1300

Sigma-Aldrich

Canine MDCKII Wild Type Cells

canine

Synonyme(s) :

MDCK II, MDCK-2, MDCKII-WT

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

product name

Canine MDCKII Wild Type Cells,

Source biologique

canine

Niveau de qualité

200

Forme

liquid

Température de stockage

−196°C

Description générale

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435).

Application

Canine MDCKII Wild Type Cells can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. See technical bulletin for detailed protocols.

Caractéristiques et avantages

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron.

Qualité

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Stockage et stabilité

Upon receiving a shipment of frozen canine MDCKII wild type cells it is important the end user gives the shipment attention without delay. To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70 °C. Storage at –70 °C will result in loss of viability.

Informations légales

Exhibit 1: ADME/Tox cell lines licenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance

Articles

CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein

Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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