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MBD0044

Sigma-Aldrich

Fungal DNA standard from Candida albicans

Suitable for PCR, sequencing and NGS, 10 ng/μL

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Concentration

10 ng/μL

Technique(s)

FISH: suitable

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

Standardization of sample analysis is essential in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing, and bioinformatics analyses.1 The human microbiome includes not only bacteria, but also viruses and fungi. While the “bacteriome” field has been researched extensively in the past years, fungi including yeasts, comprising the “mycobiome”, remain relatively neglected. Fungi and yeasts have been shown to be involved in illness conditions such as cancer and gastrointestinal disease. The human gut is populated by three fungal phyla, Ascomycota, Basidiomycota, and Zygomycota.
The fungal microbial genomic DNA standard from Candida albicans can serve as standard for benchmarking the performance along the workflow of microbiome research and metagenomic analyses, as well as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Candida albicans is an opportunistic human fungal pathogen belonging to Ascomycota. It exists as a harmless commensal in the gastrointestinal and genitourinary tracts in about 70% of humans and about 75% of women suffer from Candida infection at least once in their lifetime. C. albicans has emerged as a model organism for studying fungal pathogens along with other two fungi: Aspergillus fumigatus and Cryptococcus neoformans.2


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable as a standard for PCR, Sanger and next generation sequencing (NGS).

Caractéristiques et avantages

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, Sanger sequencing and 18S NGS
  • Improves bioinformatics analyses
  • Increases reproducibility and repeatability
  • Identity and purity of the genomic Candida albicans DNA has been confirmed by 18S NGS and gel electrophoresis

Forme physique

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

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Articles

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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