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MAK451

Sigma-Aldrich

Glucose-6-Phosphate Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

Synonyme(s) :

G6PD Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

100

Entrée

plasma
serum
tissue
cell culture medium

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

hematological disorder, anemia

Température de stockage

−20°C

Description générale

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

Application

The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

Caractéristiques et avantages

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

Adéquation

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

Principe

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Autres remarques

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Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H341

Classification des risques

Muta. 2

Code de la classe de stockage

12 - Non Combustible Liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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