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Principaux documents

MAK433

Sigma-Aldrich

Adenosine Quantification Assay Kit

sufficient for 100 fluorometric tests

Synonyme(s) :

Adenosine Test Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Utilisation

sufficient for 100 fluorometric tests

Entrée

plasma

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cancer; neurological disorders; cardiovascular diseases

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Adenosine is a purine nucleoside base, which is the precursor of adenosine triphosphate, or ATP, and is utilized thoroughly throughout the entire body in general metabolism. Extracellular adenosine acts as a local modulator with a generally cytoprotective function in the body. Its effects on tissue protection and repair fall into four categories: increasing the ratio of oxygen supply to demand; protecting against ischaemic damage by cell conditioning; triggering anti-inflammatory responses; and the promotion of angiogenesis.

Application

The Adenosine Quantification Assay Kit may be used for:
  • Cancer Research
  • Cardiovascular Disease Research
  • Neurological Disease Research

Caractéristiques et avantages

  • Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 100 tests

Adéquation

The kit is suitable for the quantification of adenosine in plasma.

Principe

The Adenosine Quantification Assay Kit provides a simple and quick fluorometric method for the detection of adenosine. The kit is based on a multi-step enzymatic approach, resulting in the formation of fluorescent product that is measured at λEx = 550 nm/λEm = 585. The kit has a linear range of 0 to 20 μM.

Autres remarques

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Code de la classe de stockage

10 - Combustible liquids

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Adenosine receptors as therapeutic targets.
Jacobson K A, et al.
Nature Reviews. Drug Discovery, 5(3), 247-264 (2006)
B B Fredholm et al.
Pharmacological reviews, 53(4), 527-552 (2001-12-06)
Four adenosine receptors have been cloned and characterized from several mammalian species. The receptors are named adenosine A(1), A(2A), A(2B), and A(3). The A(2A) and A(2B) receptors preferably interact with members of the G(s) family of G proteins and the
Monika Samsel et al.
Postepy higieny i medycyny doswiadczalnej (Online), 67, 1189-1203 (2014-01-01)
Adenosine is an endogenous purine nucleoside that plays an important role in many biochemical processes. It acts through the four types of adenosine receptors: A1, A2A, A2B, A3 which are located i.a. in the immune, nervous, circulatory, respiratory or urinary

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