MAK171
Colorimetric Biotin Assay Kit
sufficient for 200 colorimetric assays
Synonyme(s) :
Biotin Quantitation Kit
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About This Item
Produits recommandés
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
dermatological diseases; neurological disorders
Conditions d'expédition
dry ice
Température de stockage
−20°C
Catégories apparentées
Description générale
Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.
Adéquation
Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution
Principe
This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.
Notes préparatoires
Storage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Code de la classe de stockage
10 - Combustible liquids
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Les clients ont également consulté
Site-specific biotinylation of purified proteins using BirA.
Fairhead M and Mark H
Site-Specific Protein Labeling, 171-184 (2015)
High-throughput biotinylation of proteins.
High Throughput Protein Expression and Purification, 185-198 (2009)
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