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MAK087

Sigma-Aldrich

Acid Phosphatase Activity Fluorometric Assay Kit

sufficient for 500 fluorometric tests

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About This Item

Numéro CE :
Code UNSPSC :
12352200

Utilisation

sufficient for 500 fluorometric tests

Méthode de détection

fluorometric

Température de stockage

−20°C

Informations sur le gène

Description générale

The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the detection of acid phosphatase in serum and other biological samples.

Principe

The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.

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Code de la classe de stockage

12 - Non Combustible Liquids


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Significance of increased phosphatase activity of bone at the site of osteoplastic metastases secondary to carcinoma of the prostate gland.
Gutman E B, et al.
Cancer Research, 28(3), 485-495 (1936)
Kapil D Patel et al.
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Providing appropriate biophysical and biochemical cues to the interface is a facile strategy to enhance the osteogenic ability of metallic implants. Here we exploited this through the incorporation of mesoporous bioactive glass nanoparticles (MBGN) at a high content (1:1 by
Ruibin Kuang et al.
Plant physiology, 151(1), 199-209 (2009-07-28)
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with
Gaurav Datta et al.
Frontiers in cellular neuroscience, 13, 329-329 (2019-08-06)
Human immunodeficiency virus type 1 (HIV-1) associated neuropathy is the most common neurological complication of HIV-1, with debilitating pain affecting the quality of life. HIV-1 gp120 plays an important role in the pathogenesis of HIV neuropathy via direct neurotoxic effects

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