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MAK060

Sigma-Aldrich

Fumarate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Catégories apparentées

Description générale

Fumarate is a tricarboxylic acid (TCA) cycle intermediate formed by the oxidation of succinate by succinate dehydrogenase. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. Deficiencies in fumarate hydratase are associated with increased cellular concentrations of fumarate and increased incidences of renal cell cancer. This is most likely due to fumarate-mediated upregulation of HIF1. The Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples.

Application

Fumarate Assay Kit has been used to determine the concentration of fumarate in samples.

Adéquation

Suitable for the detection of fumarate in a variety of samples including tissue, cells and serum

Principe

Fumarate is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the fumarate present.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

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Combined effects of FH (E404D) and ACOX2 (R409H) cause metabolic defects in primary cardiac malignant tumor.
Zhou X, et al.
Cell Death & Disease, 5(1), 18-18 (2018)
Yijing Zhuang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 39(4), 1307-1316 (2016-09-09)
Motility is a feature of many pathogens that contributes to the migration and dispersion of the infectious agent. Whether gentamycin has a post-antibiotic effect (PAE) on the swarming and swimming motility of Escherichia coli (E. coli) remains unknown. In this
Melatonin alleviates adipose inflammation through elevating α-ketoglutarate and diverting adipose-derived exosomes to macrophages in mice.
Liu Z, et al.
Journal of Pineal Research, 64(1), e12455-e12455 (2018)
Meixi Peng et al.
Cell death & disease, 10(3), 228-228 (2019-03-10)
Citrate, a substance being related to de novo fatty acid synthesis and tricarboxylic acid (TCA) cycle, has a pivotal role in cell survival. However, the molecular mechanisms that regulate intracellular citrate in triple-negative breast cancer (TNBC), especially under hypoxic condition
Ping Li et al.
International journal of molecular sciences, 23(3) (2022-02-16)
The FH gene encodes the fumarate hydratase of the Krebs cycle and functions as a homotetramer to catalyze the hydration of fumarate to malate. Mutations in FH result in uterine leiomyomas, a rare autosomal dominant inherited metabolic disease. However, how
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Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

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