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MAK013

Sigma-Aldrich

Glucose and Sucrose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Glucose and Sucrose Colorimetric/Fluorometric Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
food and beverages

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes

Température de stockage

−20°C

Description générale

Sucrose is a disaccharide that is hydrolyzed to glucose and fructose by invertase. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Upon cellular uptake, glucose can be shunted towards breakdown via glycolysis or polymerized for energy storage.

Application

Glucose and sucrose assay kit has been used to:
  • determine sucrose, aerobic glycolysis and pyruvate kinase activity
  • determine protein and sugar in strawberries
  • determine plasma insulin and glucose levels in mice
  • analyze plasma glucose and plasma lactate and to quantify glucose/sucrose.

Adéquation

Suitable for use with various biological samples including serum, plasma, other body fluids, food, and growth media.

Principe

In this assay kit, glucose is oxidized via glucose oxidase resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the glucose present. To measure sucrose, invertase is added to the reaction to convert the sucrose to glucose and fructose. The free glucose can be subtracted from the total glucose to give the concentration of sucrose present. This kit has a linear range of detection between 0.2-1.0 nmole of glucose for the fluorometric assay and 2-10 nmoles of glucose for the colorimetric assay.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334,H412

Conseils de prudence

P261 - P273 - P284 - P501

Classification des risques

Aquatic Chronic 3 - Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

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Protocoles

Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)

Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.

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