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MAK007

Sigma-Aldrich

Asparaginase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Asparaginase Test Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

cancer

Température de stockage

−20°C

Description générale

Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartate. While asparaginase is synthesized by plants, microorganisms, and some animals, it does not occur naturally in humans. While most cells have the ability to synthesize asparagine, many hematopoietic cells do not and depend on exogenous asparagine for protein synthesis. Treatment of certain hematopoietic malignancies such as acute lymphoblastic leukemia (ALL) with asparaginase results in depletion of blood levels of asparagine resulting in cell cycle arrest and apoptosis. Asparaginase is also used in the food industry to reduce the formation of acrylamide in starchy and fried foods.

Adéquation

Suitable for measuring asparaginase activity in biological samples

Principe

The Asparaginase Activity Assay Kit provides a simple and direct procedure for measuring Asparaginse activity in a variety of biological samples. Asparaginase activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate generated. One milliunit (mU) of Asparaginase is defined as the amount of enzyme that catalyzes the formation of 1.0 mmole of aspartate per minute at 25 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

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An inhibitor of human asparagine synthetase suppresses proliferation of an L-asparaginase-resistant leukemia cell line.
Gutierrez J A, et al.
Chemistry and Biology, 13(1), 1339-1347 (2006)
Current applications and different approaches for microbial l-asparaginase production.
Cachumba J J M, et al.
Brazilian Journal of Microbiology, 47, 77-85 (2016)
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
.Zhang J, et al.
Molecular Cell, 56(2), 205-218 (2014)
Semi-permeable coatings fabricated from comb-polymers efficiently protect proteins in vivo.
Liu M, et al.
Nature Communications, 5, 5526-5526 (2014)
Yalin Zhang et al.
The American journal of Chinese medicine, 47(5), 1133-1147 (2019-07-18)
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Articles

Enzymatic Asparaginase Activity Assay

This assay protocol is suitable for the colorimetric/fluorometric detection of Asparaginase activity in bacteria, cell and tissue culture supernatants, plant samples, biological samples, and food samples using the Asparaginase Activity Assay Kit (MAK007).

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