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M8159

Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse

clone MAPK-YT, ascites fluid

Synonyme(s) :

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160410
Nomenclature NACRES :
NA.44

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

MAPK-YT, monoclonal

Poids mol.

antigen ERK-1 44 kDa
antigen ERK-2 42 kDa

Contient

15 mM sodium azide

Espèces réactives

human, Caenorhabditis elegans, Xenopus, Drosophila, hamster, rat, bovine, mouse, yeast

Technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:10,000 using rat brain extract

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

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Description générale

MAP kinase (MAPK, mitogen-activated protein kinase is also termed as ERK, extracellular regulated protein kinase). Molecular cloning has established that MAP kinase (ERKs) consists of at least four isoforms: ERK-1 (p44mapk), ERK-2 (p42mapk), ERK-3, and ERK-5. MAP kinase isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung and kidney. It is also expressed at high levels in PC12 cells and in fibroblasts. MAPK1 is located on human chromosome 22q11. MAPK3 is mapped to human chromosome 16p11.

Spécificité

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Immunogène

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

Application

Antibodies that react specifically with the active form of MAP kinase are useful for the study of the specific activation requirements, differential tissue expression, and intracellular localization of the active form of MAP kinase in normal and neoplastic tissue.
Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) may be used for the localization of the active, dually-phosphorylated, form of MAP kinase using various immunochemical assays such as immunoblotting of cultured cells and tissue extracts, ELISA, immunocytochemistry, immunoprecipitation, and in immunohistochemistry (formalin and formaldehyde-fixed sections). Reactivity has been observed with human, bovine, rat, mouse, Drosophila, Spodoptera frugiperda, and yeast.

Monoclonal Anti-MAP Kinase, activated (Diphosphorylated ERK-1&2) antibody has been used in plasmid and transient transfection and western blotting.

Actions biochimiques/physiologiques

MAP kinase (MAPK, mitogen-activated protein kinase) plays a crucial role in various signal transduction pathways leading signals of growth factor, as well as G protein-coupled receptors to their intracellular targets. MAP kinase was shown to regulate several cellular processes among them proliferation, differentiation, cellular morphology and oncogenesis. Activation of ERK-1 and ERK-2 in mitogen-stimulated cells is directly mediated by MAP kinase kinase (MAPKK or MEK), a dual-specificity protein kinase, which phosphorylates both threonine and tyrosine residues in the regulatory sites of MAP kinase. Following activation, MAP kinase phosphorylates several nuclear targets, including transcription factors as well as membrane and cytoskeletal proteins. Termination of MAP kinase signalling appears to be mediated by MAP kinase phosphatase, MKP-1, a dual specificity Thr/Tyr phosphatase which dephosphorylates and inactivates MAP kinase.

Forme physique

The product is provided as ascites fluid containing 15 mM sodium azide as a preservative.

Stockage et stabilité

Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Tetsuya Okuyama et al.
The Journal of biological chemistry, 285(39), 30274-30281 (2010-07-14)
It has not been determined yet whether the ERK-MAPK pathway regulates longevity of metazoans. Here, we show that the Caenorhabditis elegans ERK cascade promotes longevity through the two longevity-promoting transcription factors, SKN-1 and DAF-16. We find that RNAi of three
Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions.
Gray P, et al.
Endocrine Reviews, 22(2), 153-183 (2001)
The ERK cascade: distinct functions within various subcellular organelles.
Wortzel I and Seger R
Genes & Cancer, 2(3), 195-209 (2011)
Blockade of the extracellular signal-regulated kinase pathway induces marked G1 cell cycle arrest and apoptosis in tumor cells in which the pathway is constitutively activated up-regulation of p27Kip1
Hoshino R, et al.
The Journal of Biological Chemistry, 276(4), 2686-2692 (2001)
Gene expression and genotyping studies implicate the interleukin 7 receptor in the pathogenesis of primary progressive multiple sclerosis.
Booth D R, et al.
Journal of Molecular Medicine, 83(10), 822-830 (2005)

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