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M6570

Sigma-Aldrich

Monoclonal Anti-Melan-A antibody produced in mouse

clone A103, purified from hybridoma cell culture

Synonyme(s) :

Anti-MART1

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About This Item

Numéro MDL:
MFCD01633630
Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

A103, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~22 kDa

Espèces réactives

human, canine

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells ()

Isotype

IgG1

Numéro d'accès UniProt

Q16655

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MLANA(2315)

Description générale

Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors.
Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers.

Immunogène

recombinant human Melan-A protein.

Application

Anti-Melan-A antibody, Mouse monoclonal has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation
  • immunohistochemistry

Actions biochimiques/physiologiques

Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation.

Description de la cible

Melan-A is a melanoma antigen recognized by T cells.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Enayat Attwa et al.
International journal of dermatology, 54(2), 235-244 (2014-10-01)
Melasma is a common pigmentary disorder that remains resistant to available therapies. The aim of the present study was to evaluate the efficacy of erbium:YAG lasers in the treatment of refractory melasma and investigate the histopathological and ultrastructural changes between
Cytokines and Biologic Modifiers
Immunology for Pharmacy, 181-188 (2012)
A A Jungbluth et al.
Virchows Archiv : an international journal of pathology, 434(5), 429-435 (1999-07-02)
Angiomyolipomas are tumours of uncertain histogenesis, most often occurring in association with the kidney. A characteristic finding is their reactivity with HMB-45, a monoclonal antibody to the melanocyte-associated antigen gp-100. We tested 18 angiomyolipomas for their reactivity with A103, a
Y T Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5915-5919 (1996-06-11)
Recent progress in the structural identification of human melanoma antigens recognized by autologous cytotoxic T cells has led to the recognition of a new melanocyte differentiation antigen, Melan-A(MART-1). To determine the properties of the Melan-A gene product, Melan-A recombinant protein
Lin Hou et al.
Theranostics, 8(14), 3781-3796 (2018-08-08)
Rationale: Tumor-associated fibroblasts (TAFs) play a critical role in the suppressive immune tumor microenvironment (TME), compromising the efficacy of immunotherapy. To overcome this therapeutic hurdle, we developed a nanoemulsion (NE) formulation to deliver fraxinellone (Frax), an anti-fibrotic medicine, to TAFs
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