M3024

Milieu essentiel minimum d′Eagle

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

• Synonyme(s) : MEM
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.75

Propriétés

• Niveau de qualité: 500
• Forme: powder
• Technique(s): cell culture | mammalian: suitable
• Composants: Earle’s salts (5% CO₂): yes; L-glutamine: no; HEPES: no; NaHCO₃: no; sodium pyruvate: no; phenol red: no
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used:

• to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
• to culture MCF-7 cells for experimental treatments with hormone
• to maintain zebrafish pineal glands for action spectrum analysis

Caractéristiques et avantages

Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells

Quantité

Formulated to contain 9.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable