M042030

Biotin Phosphoramidite

configured for ABI

• Synonyme(s) : Biotin amidite
• Formule empirique (notation de Hill): C₄₆H₆₄N₅O₈PS
• Numéro CAS: 1217500-22-5
• Poids moléculaire : 878.07
• Numéro MDL: MFCD00676535
• Code UNSPSC : 12352200
• ID de substance PubChem : 329817780
• Nomenclature NACRES : NA.21

Propriétés

• Gamme de produits: Proligo Reagents
• Niveau de qualité: 200
• Pureté: ≥96% (³¹P-NMR)
• Adéquation: conforms to structure for H-NMR
• Compatibilité: configured for ABI
• Température de stockage: −20°C
• Chaîne SMILES: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• Clé InChI: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

Description

Description générale

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

Informations légales

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable