L9277

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonyme(s) : Anti-LIF
• Numéro MDL: MFCD00212999
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: goat
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: lyophilized powder
• Espèces réactives: human
• Technique(s): neutralization: suitable
• Numéro d'accès UniProt: P15018
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LIF(3976)

Description

Description générale

Leukemia inhibitory factor (LIF) is a pleotrophic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes human leukemia inhibitory factor. It shows no cross-reactivity with recombinant mouse LIF.

Spécificité

The antibody neutralizes the biological activity of recombinant human LIF.

Immunogène

recombinant human LIF expressed in E. coli

Application

Anti-Leukemia Inhibitory Factor antibody may be for immunoblotting a concentration of 1 μg/ml For ELISA a working concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions. For neutralization of LIF in SEK1, melanoma cell line, a concentration of 100 μg/ml was used.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Notes préparatoires

Purified using protein G

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)