L8262
Lectin from Ulex europaeus (gorse, furze)
biotin conjugate, lyophilized powder
Synonyme(s) :
Ulex europaeus agglutinin, UEA-I
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Conjugué
biotin conjugate
Niveau de qualité
Forme
lyophilized powder
Puissance
<50 μg/mL agglutination activity
Composition
Protein, ~85% E1%/280
Ampleur du marquage
~3 mol biotin per mol protein
Température de stockage
2-8°C
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Application
Lectin from Ulex europaeus (gorse, furze) has been used:
- as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
- in double staining for endothelial cells for quantitation of blood vessels
- in histochemical staining of bronchial epithelial cells
Actions biochimiques/physiologiques
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Conditionnement
Package size based on protein content.
Liaison
Conjugates are prepared from affinity purified lectin.
Forme physique
Contains sodium citrate
Remarque sur l'analyse
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
The aggregation of lymphoid cells in the bronchial mucosa has been named the bronchus-associated lymphoid tissue (BALT) and investigated in comparison with the gut-associated lymphoid tissue (GALT). To elucidate precisely the structure and function of the BALT, the present study
Michael Spencer et al.
Diabetes, 62(5), 1709-1717 (2013-01-19)
Fish oils (FOs) have anti-inflammatory effects and lower serum triglycerides. This study examined adipose and muscle inflammatory markers after treatment of humans with FOs and measured the effects of ω-3 fatty acids on adipocytes and macrophages in vitro. Insulin-resistant, nondiabetic
Sarah Waters et al.
Acta neuropathologica communications, 9(1), 144-144 (2021-08-28)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease involving progressive degeneration of upper and lower motor neurons. The pattern of lower motor neuron loss along the spinal cord follows the pattern of deposition of phosphorylated TDP-43 aggregates. The blood-spinal
Molly E V Swanson et al.
Scientific reports, 10(1), 11693-11693 (2020-07-18)
Current immunohistochemical methods of studying microglia in the post-mortem human brain do not capture the heterogeneity of microglial function in response to damage and disease. We therefore investigated the expression of eight myeloid cell proteins associated with changes in function
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