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L3772

Sigma-Aldrich

Leptin from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonyme(s) :

OB

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About This Item

Numéro CAS:
181030-10-4
Numéro MDL:
MFCD01094206
Code UNSPSC :
51111800
Nomenclature NACRES :
NA.32

Source biologique

mouse

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Stérilité

non-sterile

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤1.0 ng/mL ED50

Poids mol.

predicted mol wt ~16 kDa

Conditions de stockage

OK to freeze

Technique(s)

cell based assay: suitable

Impuretés

≤1.0 EU/μg endotoxin

Numéro d'accès UniProt

P41160

Conditions d'expédition

ambient

Température de stockage

−20°C

Chaîne SMILES 

CC(C)CC(C(=NC(CCC(=N)O)C(=NCC(=NC(CO)C(=NC(CC(C)C)C(=NC(CCC(=N)O)C(=NC(CC(=O)O)C(=NC(CCSC)C(=NC(CC(C)C)C(=NC(CC1=CNC2=CC=CC=C21)C(=NC(CCC(=N)O)C(=NC(CC(C)C)C(=NC(CC(=O)O)C(=NC(CC(C)C)C(=NC(CO)C(=O)N3CCCC3C(=NCC(=NC(CS)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N

InChI

1S/C87H138N22O28S2/c1-41(2)27-48(88)72(121)97-50(18-21-65(89)112)73(122)93-36-68(115)95-61(38-110)84(133)104-54(28-42(3)4)77(126)98-52(20-23-67(91)114)75(124)106-59(33-70(117)118)82(131)100-53(24-26-139-11)76(125)102-55(29-43(5)6)78(127)105-58(32-46-35-92-49-16-13-12-15-47(46)49)81(130)99-51(19-22-66(90)113)74(123)101-56(30-44(7)8)79(128)107-60(34-71(119)120)83(132)103-57(31-45(9)10)80(129)108-62(39-111)86(135)109-25-14-17-64(109)85(134)94-37-69(116)96-63(40-138)87(136)137/h12-13,15-16,35,41-45,48,50-64,92,110-111,138H,14,17-34,36-40,88H2,1-11H3,(H2,89,112)(H2,90,113)(H2,91,114)(H,93,122)(H,94,134)(H,95,115)(H,96,116)(H,97,121)(H,98,126)(H,99,130)(H,100,131)(H,101,123)(H,102,125)(H,103,132)(H,104,133)(H,105,127)(H,106,124)(H,107,128)(H,108,129)(H,117,118)(H,119,120)(H,136,137)/t48-,50-,51-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-/m0/s1

Clé InChI

NRYBAZVQPHGZNS-ZSOCWYAHSA-N

Informations sur le gène

mouse ... Lep(16846)

Catégories apparentées

Description générale

Research area: Cell signaling

Leptin (Ob) is a non-glycosylated polypeptide hormone. It is produced primarily in adipocytes and the placenta. The structure of leptin closely resembles members of the long-chain helical cytokine family. It is composed of four antiparallel α-helices interconnected by two long crossover links and one short loop. These structural elements are arranged in a left-handed helical bundle formation, resulting in a unique two-layer packing configuration.

Application

Leptin from mouse has been used:
  • to check the effect of leptin on ovulation for treating infertility in obese mice
  • to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice
  • to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin

Actions biochimiques/physiologiques

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Leptin, an essential cytokine, serves as a signaling molecule in the reproductive system. It modulates placental functions through autocrine or paracrine signaling. Particularly in the early stages of pregnancy, leptin plays a vital role in modulating key processes including cell proliferation, protein synthesis, invasion, and apoptosis within placental cells. Deficiency of leptin may lead to the development of severe obesity and abnormalities in hematopoiesis, immunity, angiogenesis, bone formation, blood pressure regulation and reproduction. Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation.

Forme physique

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3

Remarque sur l'analyse

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Mohammad-Reza Esmaili-Nejad et al.
Iranian journal of basic medical sciences, 18(12), 1176-1182 (2016-02-16)
Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term
P Pallares et al.
Theriogenology, 73(8), 1088-1095 (2010-02-23)
The goal of the current was to elucidate if treatment with gonadotrophins and leptin can circumvent infertility in obese mice and to establish whether reproductive effects of leptin are influenced at the hypothalamus-hypophysis or ovarian level by using a leptin
Jordan T White et al.
Hormones and behavior, 66(1), 169-179 (2014-03-04)
This article is part of a Special Issue "Energy Balance". Natural populations display a variety of reproductive responses to environmental cues, but the underlying physiology that causes these responses is largely unknown. This study tested the hypothesis that heritable variation
Karl J Kaiyala et al.
PloS one, 10(3), e0119391-e0119391 (2015-03-11)
Survival of free-living animals depends on the ability to maintain core body temperature in the face of rapid and dramatic changes in their thermal environment. If food intake is not adjusted to meet the changing energy demands associated with changes
Mark A Rossi et al.
Neuron, 109(23), 3823-3837 (2021-10-09)
The lateral hypothalamic area (LHA) regulates feeding- and reward-related behavior, but because of its molecular and anatomical heterogeneity, the functions of defined neuronal populations are largely unclear. Glutamatergic neurons within the LHA (LHAVglut2) negatively regulate feeding and appetitive behavior. However
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