L3001

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg solid

• Synonyme(s) : Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numéro CAS: 9001-62-1
• Numéro de classification (Commission des enzymes): 3.1.1.3 (BRENDA, IUBMB)
• Numéro CE : 232-619-9
• Numéro MDL: MFCD00131509
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: wheat (germ)
• Type: Type I
• Forme: lyophilized powder
• Activité spécifique: 5-15 units/mg solid
• Numéro d'accès UniProt: Q8L6B0
• Température de stockage: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clé InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N
• Informations sur le gène: wheat ... lipase(543006)

Description

Description générale

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:

• to prepare enzyme solution for the biodegradability test
• to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
• to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Actions biochimiques/physiologiques

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Qualité

Contains acid phosphatase.

Définition de l'unité

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO₂ in 30 minutes.)

Notes préparatoires

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)