L1667

Lysozyme human

recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder

• Synonyme(s) : Lysobac^™ r Lysozyme, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• Numéro CAS: 12671-19-1
• Numéro de classification (Commission des enzymes): 3.2.1.17 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131557
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in rice
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized powder
• Activité spécifique: ≥100,000 units/mg protein
• Poids mol.: 16.5 kDa
• Technique(s): cell based assay: suitable
• Adéquation: suitable for cell lysis
• Numéro d'accès UniProt: P61626
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... LYZ(4069)

Description

Description générale

Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.

Application

Lysozyme human has been used:

• in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
• in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
• in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)

Actions biochimiques/physiologiques

Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.

Caractéristiques et avantages

Lysobac^™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac^™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:

• Improves Bacterial Fermentation Efficiency
• Greater bacterial lysis activity per mg.
• 1 gram of delivers more lysis activity than 4 grams of HEWL
• Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
• Improved recombinant protein yield compared to mechanical lysis
• Reduces cost of goods

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will produce a ΔA₄₅₀ of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations légales

Lysobac is a trademark of InVitria

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable