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Key Documents

L0770

Sigma-Aldrich

Lectin from Pisum sativum (pea)

FITC conjugate, lyophilized powder

Synonyme(s) :

Pisum sativum agglutinin, PSA

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Conjugué

FITC conjugate

Niveau de qualité

Forme

lyophilized powder

Puissance

<20 μg per mL agglutination activity

Composition

Protein, ≥95% biuret

Ampleur du marquage

2-4 mol FITC per mol protein (Lowry).

Impuretés

salt, free

Température de stockage

−20°C

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Description générale

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Actions biochimiques/physiologiques

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).
PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Notes préparatoires

Conjugates are prepared from affinity purified lectin.

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Crypticity and functional distribution of the membrane associated ?-L-fucosidase of human sperm
Venditti JJ, et al.
Molecular Reproduction and Development, 74(6), 758-766 (2007)
Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria
Purish LM, et al.
Applied Biochemistry and Microbiology, 49(5), 458-463 (2013)
Amar K Chandra et al.
Environmental toxicology, 32(6), 1823-1835 (2017-02-17)
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress
In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
Tácia Gomes Bergstein-Galan et al.
Theriogenology, 96, 69-75 (2017-05-24)
This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis.

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