I1884
Insulin-transferrin-sodium selenite media supplement
γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
Synonyme(s) :
ITS Supplement
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About This Item
Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75
Produits recommandés
Description générale
Insulin-transferrin-sodium selenite media supplement supports in vitro growth and maturation of follicles from oocyte and is routinely used in in vitro fertilization methods.
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
Application
Insulin-transferrin-sodium selenite media supplement has been used as:
- medium 199 (M199) media supplement for cardiac myocytes culture
- Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture
- Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells
Autres remarques
Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Quantité
Each vial sufficient to prepare 5 liters of medium.
Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
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