Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

I1408

Sigma-Aldrich

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Synonyme(s) :

Affinity Gel

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

(1:1 suspension in a 30% ethanol solution)

Durée de conservation

1 yr

Technique(s)

affinity chromatography: suitable

Impuretés

≤0.08 Metal Contamination (μmol/ml packed gel)

Matrice

6% beaded agarose

Capacité

10-30 μmol/mL binding capacity (Cu+2)

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Application

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

Forme physique

1:1 suspension in a 30% ethanol solution

Notes préparatoires

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Autres remarques

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

120.2 °F - closed cup

Point d'éclair (°C)

49 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Hao Li et al.
Proteomics, 9(6), 1646-1661 (2009-03-03)
Ethylene regulates a variety of stress responses and developmental adaptation in plants. In the present study, the phosphoproteomics is adopted to investigate the differential protein phosphorylation by ethylene in Arabidopsis ethylene-insensitive 2 (ein2) mutant. A total of 224 phosphopeptides were
The hantavirus glycoprotein G1 tail contains dual CCHC-type classical zinc fingers
Estrada DF, et al.
The Journal of Biological Chemistry, 284(13), 8654-8660 (2009)
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of Biological Chemistry, jbc-M111 (2012)
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of biological chemistry, jbc-M111 (2012)
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
Test, jbc-M111 (2012)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique