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Key Documents

HCLHER2DR

Sigma-Aldrich

HER2 Analyte ControlDR

Cell line controls for immunohistochemistry and in situ hybridization

Synonyme(s) :

HER2 Dynamic Range Analyte Control, Human Epidermal growth factor Receptor Analyte Control Dynamic Range

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.47

Source biologique

human cell culture

Conditionnement

pkg of 1 ea cell microarray block (HCL028)
pkg of 2 slides (HCL026)
pkg of 5 slides (HCL027)

Fabricant/nom de marque

Histocyte

Température de stockage

2-8°C

Catégories apparentées

Description générale

The HER2 Analyte ControlDR has four cell line cores which demonstrate a full range of expression for HER2. The cell lines selected incorporate HER2 expression levels of 0, 1+ (both non-amplified), 2+ (equivocal), and 3+ (amplified).

HistoCyte Laboratories control slides and blocks are made from cultured human cell lines to provide the required range of expression of a specific biomarker. The cell lines are processed using proprietary methods that allow for the creation of a high density embedded cell block with cores that retain their cellular morphology. Available as pre-cut unstained slides or as cell microarray blocks for sectioning.

Application

Ideal for use as a same slide control for immunohistochemistry (IHC) and in situ hybridization (ISH). This control has been developed for researchers who require a reliable, sensitive and accurate control for HER2.
The HER2 Analyte ControlDR histology slides have four cell line cores providing a dynamic range of expression of HER2. Analyte controls are placed at one end of slide, allowing sample application on same slide for valid comparison.
The HER2 Analyte ControlDR cell microarray block contains four cell line cores that are 2 mm in diameter and between 3.0-3.5 mm in length. The cell microarray block may yield over 300 sections depending on thickness.

Notes préparatoires

Cell microarray blocks are fixed in 10% neutral buffered formalin and embedded in paraffin wax. Pre-cut unstained slides are prepared from 4 μm thick sections, mounted on positively charged slides, and dried at 37 °C overnight.

Autres remarques

For certificates of analysis, contact info@histocyte.com.
HER2 Analyte ControlDR brochure is available from HistoCyte Laboratories.

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