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H7540

Sigma-Aldrich

Anti-Huntingtin (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-HD, Anti-HTT, Anti-Huntington Disease, Anti-IT15

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~110 kDa

Espèces réactives

mouse, human, rat

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

Concentration

~1.5 mg/mL

Technique(s)

western blot: 0.5-1.0 μg/mL using a HEK-293T cell lysate expressing a N-terminal fragment of human huntingtin

Numéro d'accès UniProt

P42858

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HTT(3064)
mouse ... Htt(15194)
rat ... Htt(29424)

Description générale

Anti-Huntingtin (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human huntingtin, conjugated to KLH.

Application

Anti-Huntingtin (N-terminal) antibody has been used:
  • in magnetic bead-assisted immunopurification
  • in western blotting
  • in immunofluorescent staining
  • in immunoblotting

Actions biochimiques/physiologiques

Huntingtin (Htt) is essential for embryonic development and neurogenesis. Htt associates with various cell organelles like the nucleus, endoplasmic reticulum and Golgi complex. It is also found in neurites and synapses where it associates with vesicular structures and microtubules, suggesting that it may play a role in intracellular vesicular trafficking, calcium homeostasis, neuronal survival, morphogenesis and transcriptional regulation. Aberrant expansion of glutamine repeats (polyQ) in the N-terminal region of huntingtin (htt) causes Huntington′s disease (HD) which is an autosomal dominant neurodegenerative disorder.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Huntingtin-protein interactions and the pathogenesis of Huntington's disease
Li SH and Li XJ
Trends in Genetics, 20(3), 146-154 (2004)
Wojciech J Szlachcic et al.
Frontiers in molecular neuroscience, 10, 253-253 (2017-08-30)
Huntington disease (HD) is an incurable neurodegenerative disorder caused by expansion of CAG repeats in huntingtin (HTT) gene, resulting in expanded polyglutamine tract in HTT protein. Although, HD has its common onset in adulthood, subtle symptoms in patients may occur
Prefibrillar huntingtin oligomers isolated from HD brain potently seed amyloid formation
Morozova O A, et al.
Febs Letters, 589(15), 1897-1903 (2015)
Luisa Massai et al.
BMC biochemistry, 14, 34-34 (2013-11-28)
Huntington's disease (HD) is a monogenic disorder caused by an aberrant expansion of CAG repeats in the huntingtin gene (HTT). Pathogenesis is associated with expression of the mutant (mHTT) protein in the CNS, with its levels most likely related to
Normal huntingtin function: an alternative approach to Huntington's disease
Cattaneo E, et al.
Nature Reviews. Neuroscience, 6(12), 919-919 (2005)
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