Accéder au contenu
Merck
Toutes les photos(5)

Key Documents

View All Documentation

H2663

Sigma-Aldrich

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal

clone HDAC2-62, purified from hybridoma cell culture

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(5)

About This Item

Numéro MDL:
MFCD02096544
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

HDAC2-62, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~55 kDa

Espèces réactives

human, chicken, bovine, canine, rat, mouse

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extracts from NIH3T3 cells

Isotype

IgG2b

Numéro d'accès UniProt

Q92769

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HDAC2(3066)
mouse ... Hdac2(15182)
rat ... Hdac2(84577)

Description générale

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the HDAC2-62 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human histone deacetylase 2 (amino acid residues with N-terminal added cysteine) conjugated to maleimide-activated KLH. Histone deacetylase 2 (HDAC2) is the catalytic subunits of different multiprotein regulatory complexes.

Spécificité

Monoclonal Anti-Histone Deacetylase 2 (HDAC2) recognizes human, bovine, dog, rat, mouse, and chicken HDAC2 (approx. 55 kDa). The antibody epitope resides within amino acids 471-488 of human HDAC2.

Application

Anti-Histone Deacetylase 2 (HDAC2) antibody, Mouse monoclonal has been used in:
  • co-immunoprecipitation
  • chromatin immunoprecipitation (ChIP)
  • western blot
  • immunohistochemistry
  • enzyme linked immunosorbent assays (ELISA)

Actions biochimiques/physiologiques

Histone Deacetylases including HDAC′s 1-10 facilitate transcriptional repression and nucleosome condensation. Abnormal recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. Inhibition of HDAC2 activity by valproic acid induces proteasomal degradation of HDAC2.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dimethyl fumarate regulates histone deacetylase expression in astrocytes
Kalinin S, et al.
Journal of Neuroimmunology, 263 (2013)
Jeyran Shahbazi et al.
Oncotarget, 5(12), 4257-4268 (2014-06-24)
Myc oncoproteins and histone deacetylases (HDACs) exert oncogenic effects by modulating gene transcription. Paradoxically, N-Myc induces p53 gene expression. Tumor protein 53-induced nuclear protein 1 (TP53INP1) phosphorylates p53 protein at serine 46, leading to enhanced p53 activity, transcriptional activation of
Histone Deacetylases: A Saga of Perturbed Acetylation Homeostasis in Cancer
Parbin S, et al.
The Journal of Histochemistry and Cytochemistry, 62 (2014)
Insights into the activation mechanism of class I HDAC complexes by inositol phosphates
Watson PJ, et al.
Nature Communications, 7 (2016)
Stem Cell Factor SALL4 Represses the Transcriptions of PTEN and SALL1 through an Epigenetic Repressor Complex
Lu J, et al.
Testing, 4 (2009)
Afficher tous les articles scientifiques apparentés

Articles

Huntington's Disease

Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction, motor impairment and behavioral changes.

Contenu apparenté

ChIP Tested Antibodies

Chromatin structure plays a fundamental role in regulating processes that take place on the DNA, such as transcription, replication, and recombination, all of which occur on nucleosomal templates. Therefore, mapping the position of histones selectively modified and histone variants or non-histone components of chromatin in specific DNA sequences, can provide valuable insights into how these proteins (and their modifications) function in a chromatin context.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique