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GE28-9031-30

Streptavidin High Performance Spintrap™

Cytiva 28-9031-30, pack of 16 columns

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.25

Caractéristiques

wetted part: no

Conditionnement

pack of 16 columns

Fabricant/nom de marque

Cytiva 28-9031-30

Conditions de stockage

(20% Ehtanol)
 (20% Ethanol)

Volume du lit

800 μL

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-10.5

working range

4-9

Capacité

>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium)
 binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

Température de stockage

2-8°C

Description générale

Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.

Application

Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.

Caractéristiques et avantages

  • For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
  • Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
  • Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
  • Easy scale-up with HiTrap® Streptavidin HP prepacked columns.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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