GE28-4082-61

HiTrap^® MabSelect^™ Xtra

Cytiva 28-4082-61, pack of 5 × 5 mL

• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: recombinant protein A (E. coli)
• Conditionnement: pack of 5 × 5 mL
• Fabricant/nom de marque: Cytiva 28-4082-61
• Conditions de stockage: (20% Ehtanol); (20% Ethanol)
• Paramètres: 42 psi
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: highly cross-linked agarose
• Taille moy. des particules: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• Capacité: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• Adéquation: suitable for bioprocess medium
• Température de stockage: 2-8°C

Description

Description générale

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ Xtra columns are prepacked with MabSelect^™ Xtra, a BioProcess medium for capture of monoclonal antbodies from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

The increased capacity of the medium is well suited for purification of Mabs from high-level expression feedstock.

Caractéristiques et avantages

• Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect^™ medium.
• MabSelect^™ Xtra uses the same recombinant Protein A ligand as MabSelect^™
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap^® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids