GE25-6600-30
GenomiPhi™ V2 DNA Amplification Kit, 25 RXN
Cytiva 25-6600-30, sufficient for 25 reactions
About This Item
Produits recommandés
Utilisation
sufficient for 25 reactions
Fabricant/nom de marque
Cytiva 25-6600-30
Disponibilité
not available in Japan
Conditions d'expédition
dry ice
Température de stockage
−70°C
Description générale
The illustra™ GenomiPhi™ V2 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for mini-scale whole genome amplification by isothermal strand displacement amplification.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
The illustra™ GenomiPhi™ V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 μg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA card lysates, blood and buccal swabs.
GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.
Application
Caractéristiques et avantages
- Outperforms PCR-based whole genome amplification techniques.
- Quick and simple automation-friendly protocol; no thermal cycler required.
- Representative isothermal amplification of the whole genome.
- No template-independent, background amplification product.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
Code de la classe de stockage
12 - Non Combustible Liquids
Certificats d'analyse (COA)
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Protocoles
The GenomiPhi™ method of WGA from Cytiva uses Phi29 DNA polymerase, a highly processive enzyme with excellent strand-displacement activity, in combination with random-sequence hexamer primers (random hexamers) to amplify DNA in an isothermal process—a thermal cycler is not required.
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