GE17-5131-02
GSTrap™ Fast Flow
Cytiva 17-5131-02, pack of 5 × 5 mL
Synonyme(s) :
GSTrap Column
About This Item
Produits recommandés
ligand
glutathione and 10-carbon linker arm
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 17-5131-02
Paramètres
flow rate
42 psi
Taille du lit
16 mm × 25 mm
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
highly cross-linked 4% agarose
Diamètre moyen
90 μm
cleaning
3-12
working range
3-12
Capacité
>10 mg/mL binding capacity
Catégories apparentées
Description générale
GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC™ System.
* See the section for licensing information in our terms and conditions.
Caractéristiques et avantages
- Fast and simple one-step purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Easy scale-up by connecting the columns in series.
- Sepharose™ 4 Fast Flow provides good flow properties.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Protocoles
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.
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