GE17-5123-10
benzamidin Seph 4Fast Flow H-sub
Cytiva 17-5123-10, pack of 25 mL
About This Item
Produits recommandés
ligand
p-aminobenzamidine
Conditionnement
pack of 25 mL
Fabricant/nom de marque
Cytiva 17-5123-10
Conditions de stockage
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Matrice
highly cross-linked 4% agarose
Diamètre moyen
90 μm
cleaning in place
1-9
Plage de travail
2-8
Température de stockage
2-8°C
Catégories apparentées
Description générale
Application
Caractéristiques et avantages
- Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
- p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
- For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
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Articles
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Protocoles
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
Global Trade Item Number
Référence | GTIN |
---|---|
GE17-5123-10 | 4061837621710 |
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