Accéder au contenu
Merck
Toutes les photos(1)

Documents

GE17-5087-01

Sigma-Aldrich

HiPrep™ 26/10 Desalting

Cytiva 17-5087-01, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Synonyme(s) :

Desalting Column, HiPrep Desalting Column

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

Matériaux

polypropylene (PP)

Poids mol.

1000-5000 Da (globular proteins)

Conditionnement

pkg of 1 ea

Fabricant/nom de marque

Cytiva 17-5087-01

Conditions de stockage

dry media

Paramètres

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

Volume du lit

53 mL

Colonne, L × D.I.

10 cm × 26 mm

Matrice

cross-linked dextran
polymeric

Groupe de la matrice active

dextran-epichlorohydrin phase

Taille des particules

17-132 μm (dry)

cleaning

2-13

working range

2-13

Technique de séparation

size exclusion (SEC)

Catégories apparentées

Description générale

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Caractéristiques et avantages

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Adéquation

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.

General Considerations for Purification of GST-tagged Protein

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

Afficher tout

Protocoles

Affinity Chromatography in a Purification Strategy (CIPP)

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Tag Removal by Enzymatic Cleavage

This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique