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GE17-1082-01

Phenyl Sepharose™ High Performance

Cytiva 17-1082-01, pack of 75 mL

Synonyme(s) :

Phenyl sepharose

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

phenyl

Conditionnement

pack of 75 mL

Fabricant/nom de marque

Cytiva 17-1082-01

Matrice

6% cross-linked agarose

Taille des particules

24-44 μm

Taille moy. des particules

34 μm

cleaning in place

2-14

Plage de travail

3-13

Adéquation

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

Description générale

Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

Caractéristiques et avantages

  • Aromatic HIC medium designed for intermediate purification and polishing steps
  • Selectivity similar to Phenyl Sepharose™ 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Désolés, nous n'avons pas de COA pour ce produit disponible en ligne pour le moment.

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Articles

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature

Protocoles

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from Cytiva, considering both automated and manual

Selectivity and the Properties of a HIC Medium

Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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