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GE17-0575-01

Chelating Sepharose Fast Flow

Cytiva 17-0575-01, pack of 50 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

iminodiacetic acid

Durée de conservation

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Conditionnement

pack of 50 mL

Fabricant/nom de marque

Cytiva 17-0575-01

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

working range

3-13

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

Chelating Sepharose Fast Flow is a BioProcess IMAC medium, designed for purifying native and recombinant proteins and peptides that have an affinity for metal ions.

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

Chelating Sepharose Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

Chelating Sepharose Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Caractéristiques et avantages

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of native and recombinant proteins
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • BioProcess medium supported for industrial applications and well-established in approved processes

Stockage et stabilité

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocoles

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap™ Kit, HiTrap® Chelating HP and Chelating Sepharose® Fast Flow products from Cytiva.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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