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Key Documents

GE17-0120-01

Sepharose 4B

Cytiva 17-0120-01, pack of 1 L

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Durée de conservation

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Conditionnement

pack of 1 L

Fabricant/nom de marque

Cytiva 17-0120-01

Matrice

4% agarose

Taille des particules

45-165 μm

cleaning in place

4-9

working range

4-9

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

Clé InChI

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Catégories apparentées

Description générale

Sepharose 4B is a well-proven Agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Application

Sepharose is a bead-formed Agarose-based gel filtration matrix. Sepharose is available with 3 different Agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight


Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents

Caractéristiques et avantages

  • 4% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

Stockage et stabilité

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds
Sudhir Chandna et al.
International journal of radiation biology, 90(5), 401-406 (2014-02-18)
Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. Macrocolony formation
Yan Hong et al.
Journal of chromatography. A, 1342, 30-36 (2014-04-02)
Previously, we studied bovine serum albumin (BSA) uptake to poly(ethylenimine) (PEI)-grafted Sepharose resins, and an ionic capacity (IC) range (600-740mmol/L) for steep increases of both protein capacity (qm) and effective pore diffusion coefficient (De) was found. In this work, seven
Sieving by agarose gels and its use during pulsed-field electrophoresis.
P Serwer
Biotechnology & genetic engineering reviews, 8, 319-343 (1990-01-01)

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