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Key Documents

GE17-0090-02

Sephadex® LH-20

Cytiva 17-0090-02, pack of 500 g

Synonyme(s) :

Sephadex Gel

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About This Item

Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Caractéristiques

autoclavable 20 min at 121°C

Conditionnement

pack of 500 g

Fabricant/nom de marque

Cytiva 17-0090-02

Matrice

hydroxypropylated, cross-linked dextran

Taille des particules

163 μm (in methanol max.)
18-111 μm (dry)
27 μm (In Methanol Min.)

cleaning in place

2-13

working range

2-13

Description générale

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.

Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility.

Caractéristiques et avantages

  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Excellent batch-to-batch reproducibility
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 25 °C ()

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Packing a Column and Performing a Separation with Sephadex® LH-20

This page shows how to perform a separation with a Sephadex LH-20 column from Cytiva which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.

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