Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

G9297

Sigma-Aldrich

Glutathione Reductase human

buffered aqueous solution, ≥10 units/mg protein, recombinant, expressed in E. coli

Synonyme(s) :

GR, Glutathione-disulfide reductase, NADPH:oxidized glutathione oxidoreductase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Produit recombinant

expressed in E. coli

Niveau de qualité

Forme

buffered aqueous solution

Activité spécifique

≥10 units/mg protein

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... GSR(2936)

Description générale

Research area: Cell signaling. Glutathione Reductase belongs to the homodimericFAD−disulfide oxidoreductases family. is made up of highly conserved domains such as two Rossmann fold domains, where one binds to reduced nicotinamide adenine dinucleotide phosphate (NADPH) and flavin adenine dinucleotide (FAD) the second one is an interface dimerization domain. It favors its accumulation in the regions of high electron flux in cells where reactive species are generated.

Application

Human glutathione reductase has been used in:
  • glutathione reductase activity assay
  • oxidative stress analysis
  • redox assays

Actions biochimiques/physiologiques

Glutathione reductase enzyme is a homodimeric enzyme containing 1 FAD molecule and 1 NADPH binding domain per subunit. Both human GR (hGR) and Plasmodium falciparum GR (PfGR) are essential for the survival of the malaria parasite within the human erythrocyte. Thus, this enzyme may be used for studies of candidate anti-malaria reagents.
Glutathione reductase is a ubiquitous flavoenzyme involved in the protection from cell stress. Glutathione reductase catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). It is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH, which serves as an antioxidant reacting with free radicals and organic peroxides. Glutathione is also an electron donor for glutathione peroxidases and a substrate for glutathione S-transferases contributing to the detoxification and elimination of toxic electrophilic metabolites and xenobiotics.
Human glutathione reductase is suitable as cytostatic and antimalarialagent. It may also be used to protect against malaria by mimicking favism by blockingthe enzyme with specific inhibitors.

Définition de l'unité

1 unit will reduce 1.0 μmole of DTNB to TNB per minute at 25 °C at pH 7.5.

Forme physique

Solution containing 25 mM Tris-HCl, pH 7.4, 1 mM EDTA, and 50% (v/v) glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Produit(s) apparenté(s)

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Haitao Shi et al.
Plant physiology and biochemistry : PPB, 71, 226-234 (2013-08-27)
As a gaseous molecule, hydrogen sulfide (H2S) has been recently found to be involved in plant responses to multiple abiotic stress. In this study, salt (150 and 300 mM NaCl), osmotic (15% and 30% PEG6000) and cold (4 °C) stress
Marcela Z Campanini et al.
Journal of photochemistry and photobiology. B, Biology, 127, 153-160 (2013-09-18)
Plants rich in antioxidant substances may be a promising strategy for preventing UV-induced oxidative and inflammatory damage of the skin. Pimenta pseudocaryophyllus is native to Brazil and presents flavonoids and other polyphenolic compounds in high concentration. Thus, the present study
Dietary methionine can sustain cytosolic redox homeostasis in the mouse liver
Sofi Eriksson, et al.
Nature Communications (2014)
C C Bohme et al.
The Journal of biological chemistry, 275(48), 37317-37323 (2000-09-02)
The homodimeric flavoenzyme glutathione reductase (GR) maintains high intracellular concentrations of the antioxidant glutathione (GSSG + NADPH + H(+) <--> 2 GSH + NADP(+)). Due to its central function in cellular redox metabolism, inhibition of GR from the malarial parasite
Walid Abu El-Soud et al.
Plant physiology and biochemistry : PPB, 71, 173-183 (2013-08-14)
Seed germination and growth of seedlings are critical phases of plant life that are adversely affected by various environmental cues. Water availability is one of the main factors that limit the productivity of many crops. This study was conducted to

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique