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G5768

Sigma-Aldrich

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Synonyme(s) :

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.72

Forme

fine powder

Niveau de qualité

200

Technique(s)

cell culture | plant: suitable

Application(s)

agriculture

Température de stockage

2-8°C

Catégories apparentées

Description générale

Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Application

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer
  • as a media to maintain potato suspension and callus cultures
  • as a media in the primary-node transformation method

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Variante de formule

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Quantité

Formulated to contain 3.1 grams of powder per liter of medium.

Pictogrammes

Flame over circle
GHS03

Mention d'avertissement

Warning

Mentions de danger

H272

Classification des risques

Ox. Sol. 3

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and
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Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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