G211200

DMT-2′Fluoro-dG(ib) Phosphoramidite

• Synonyme(s) : DMT-2′Fluoro-dG(ib) Amidite
• Formule empirique (notation de Hill): C₄₄H₅₃FN₇O₈P
• Numéro CAS: 144089-97-4
• Poids moléculaire : 857.91
• Numéro MDL: MFCD12912415
• Code UNSPSC : 41116105
• ID de substance PubChem : 329799870
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: non-animal source (no BSE/TSE risk)
• Gamme de produits: Proligo Reagents
• Pureté: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Forme: powder
• Technique(s): oligo synthesis: suitable
• Impuretés: ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (reversed phase HPLC); ≤3% residual Solvent content
• Couleur: white to off-white
• λ: conforms (UV/VIS Identity)
• Adéquation: conforms to structure for H-NMR; conforms to structure for LC-MS
• Profil des nucléosides: base: deoxyguanosine; base protecting group: isobutyryl; 2' protecting group: fluoro; 5' protecting group: DMT; deprotection: fast/standard
• Température de stockage: −20°C
• Chaîne SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)C(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C44H53FN7O8P/c1-27(2)40(53)49-43-48-39-37(41(54)50-43)47-26-51(39)42-36(45)38(60-61(58-24-12-23-46)52(28(3)4)29(5)6)35(59-42)25-57-44(30-13-10-9-11-14-30,31-15-19-33(55-7)20-16-31)32-17-21-34(56-8)22-18-32/h9-11,13-22,26-29,35-36,38,42H,12,24-25H2,1-8H3,(H2,48,49,50,53,54)/t35-,36-,38-,42-,61?/m1/s1
• Clé InChI: KJFUMXZZVYMQEU-AOCJBPQJSA-N

Description

Description générale

DMT-2′Fluoro-dG(ib) Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.

Its key features include,

• Can be employed together with DNA or RNA phosphoramidites
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
• Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
• Consistent lot-to-lot high purity and performance
• Manufactured under a certified ISO 9001 quality system

Application

DMT-2′Fluoro-dG(ib) Phosphoramidite is suitable for use in the synthesis of 2′-fluoro-modified oligonucleotide sequences using automated solid-phase chemistry.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable