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F9175

Sigma-Aldrich

Flt-3/Flk-2 Ligand from mouse

≥97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

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About This Item

Numéro MDL:
MFCD01093789
Code UNSPSC :
51111800
Nomenclature NACRES :
NA.32

Source biologique

mouse

Niveau de qualité

100

Produit recombinant

expressed in NSO cells

Pureté

≥97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

1.0-4.0 ng/mL

Poids mol.

~18 kDa

Conditionnement

pkg of 5 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Numéro d'accès UniProt

P49772

Température de stockage

−20°C

Informations sur le gène

mouse ... Flt3l(14256)

Description générale

Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor. Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.
Mouse and human Flt-3 ligand share approximately 72% homology and both demonstrate cross-species activity.

Actions biochimiques/physiologiques

Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myeloblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP and suppresses Erk signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.

Qualité

Heterogenous glycosylation causes recombinant mouse Flt-3 ligand to migrate as multiple bands from 18-32 kDa.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 50% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Remarque sur l'analyse

Bioactivity is measured by the ability to stimulate the proliferation of a Flt-3 transfected pro-B cell line.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

S D Lyman et al.
Current opinion in hematology, 2(3), 177-181 (1995-05-01)
The flt3 ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cells. Other members of this family include Steel factor (also known as mast cell growth factor, stem cell factor, and kit
Yejing Ge et al.
Journal of cell science, 126(Pt 15), 3370-3379 (2013-05-25)
Various cues initiate multiple signaling pathways to regulate the highly coordinated process of skeletal myogenesis. Myoblast differentiation comprises a series of ordered events starting with cell cycle withdrawal and ending with myocyte fusion, with each step probably controlled by multiple
Yasuyuki Saito et al.
European journal of immunology, 43(6), 1651-1658 (2013-03-23)
Lymphoid-tissue dendritic cells (DCs) are short-lived and need to be continuously replenished from bone marrow-derived DC progenitor cells. Fms-related tyrosine kinase 3 is expressed during cellular development from hematopoietic progenitors to lymphoid-tissue DCs. Fms-related tyrosine kinase 3 ligand (Flt3L) is

Articles

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

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