F6140

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse

clone FN-3E2, ascites fluid

• Synonyme(s) : Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
• Numéro MDL: MFCD00164562
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: FN-3E2, monoclonal
• Poids mol.: antigen 240 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: mouse, human, chicken
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect immunofluorescence: 1:400 using cultured chicken fibroblasts; radioimmunoassay: suitable; western blot: suitable
• Isotype: IgM
• Numéro d'accès UniProt: P02751
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FN1(2335) ; mouse ... Fn1(14268)

Description

Description générale

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Spécificité

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Immunogène

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:

• immunoblot
• immunofluorescent microscopy
• immunocytochemistry

Actions biochimiques/physiologiques

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .

Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable