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D8045

Sigma-Aldrich

AccuTaq™ LA DNA Polymerase

High fidelity Taq enzyme, with 10X buffer & DMSO

Synonyme(s) :

High Fidelity DNA polymerase, High fidelity PCR, Long range PCR

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About This Item

Numéro MDL:
MFCD01092095
Code UNSPSC :
41106314
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 125 reactions
 sufficient for 1500 reactions
 sufficient for 500 reactions

Caractéristiques

Long & Accurate PCR
dNTPs included: no
hotstart: no

Concentration

5 units/μL

Technique(s)

PCR: suitable

Couleur

colorless

Entrée

purified DNA

Adéquation

suitable for PCR

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

AccuTaq™ long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3′→5′ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end.

Application

AccuTaq™ LA DNA Polymerase produces long DNA amplicons for:
  • Mutation analysis
  • Cloning genes
  • DNA sequencing
  • cDNA library generation
  • Polymerase chain reaction (PCR)
  • Direct PCR

Caractéristiques et avantages

  • Increased fidelity, up to 6.5× that of Taq DNA polymerase
  • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.

Conditionnement

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.

Autres remarques

AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance.

Définition de l'unité

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.

Informations légales

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Daniel Petit et al.
The Journal of biological chemistry, 285(49), 38399-38414 (2010-09-22)
Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. β-Galactoside α2,6-sialyltransferases ST6Gal I and ST6Gal II are the two unique members of the ST6Gal family
Eugene J Gardner et al.
Nature communications, 10(1), 4630-4630 (2019-10-13)
Mobile genetic Elements (MEs) are segments of DNA which can copy themselves and other transcribed sequences through the process of retrotransposition (RT). In humans several disorders have been attributed to RT, but the role of RT in severe developmental disorders
Paulina Kleniewska et al.
Oxidative medicine and cellular longevity, 2013, 308358-308358 (2013-04-12)
The aim of the present study was to assess whether BAY 11-7082, a nuclear factor-kappaB (NF- κ B) inhibitor, influences the level of reactive oxygen species (ROS), tumor necrosis factor alpha (TNF- α), and NF- κ B related signaling pathways
Haim Levy et al.
Infection and immunity, 80(8), 2623-2631 (2012-05-16)
The virulence of Bacillus anthracis, the causative agent of anthrax, stems from its antiphagocytic capsule, encoded by pXO2, and the tripartite toxins encoded by pXO1. The accepted paradigm states that anthrax is both an invasive and toxinogenic disease and that
L S Waldron et al.
Applied and environmental microbiology, 75(1), 108-112 (2008-11-04)
Effective management of human cryptosporidiosis requires efficient methods for detection and identification of the species of Cryptosporidium isolates. Identification of isolates to the species level is not routine for diagnostic assessment of cryptosporidiosis, which leads to uncertainty about the epidemiology
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Protocoles

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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