D2944
Anti-D1 Dopamine Receptor antibody,Rat monoclonal
clone 1-1-F11 s.E6, purified from hybridoma cell culture
Synonyme(s) :
Anti-DRD1
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About This Item
Produits recommandés
Source biologique
rat
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
1-1-F11 s.E6, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~50 kDa
Espèces réactives
rat, monkey, human
Concentration
~1 mg/mL
Technique(s)
immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... DRD1(1812)
rat ... Drd1a(24316)
Description générale
Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.
Immunogène
recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:
- immunohistochemistry
- immunoblotting
- immunocytochemistry
- proximity ligation assay
- co-immunoprecipitation
- western blotting
Actions biochimiques/physiologiques
D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.
Description de la cible
D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Informations légales
Sold under exclusive license from Emory University
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
E Cahill et al.
Molecular psychiatry, 19(12), 1295-1304 (2014-07-30)
Convergent dopamine and glutamate signalling onto the extracellular signal-regulated kinase (ERK) pathway in medium spiny neurons (MSNs) of the striatum controls psychostimulant-initiated adaptive processes underlying long-lasting behavioural changes. We hypothesised that the physical proximity of dopamine D1 (D1R) and glutamate
Matthieu F Bastide et al.
Scientific reports, 7(1), 2348-2348 (2017-05-26)
A whole brain immediate early gene mapping highlighted the dorsolateral bed nucleus of the stria terminalis (dlBST) as a structure putatively involved in L-3,4-dihydroxyphenylalanine (L-Dopa)-induced dyskinesia (LID), the debilitating side-effects of chronic dopamine replacement therapy in Parkinson's disease (PD). dlBST
Impact of early consumption of high-fat diet on the mesolimbic dopaminergic system
Naneix F, et al.
eNeuro, 4(3) (2017)
Aliza T Ehrlich et al.
Molecular pharmacology, 84(3), 476-486 (2013-07-12)
The mechanism underlying the crosstalk between multiple G protein-coupled receptors remains poorly understood. We previously reported that prostaglandin E receptor EP1 facilitates dopamine D1 receptor signaling in striatal slices and promotes behavioral responses induced by D1 receptor agonists. Here, using
D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses
Cahill E, et al.
Molecular Psychiatry, 19(12), 1295-1295 (2014)
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