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Key Documents

CWDS-IF647

Sigma-Aldrich

ColorWheel® Antibody-Ready iFluor 647 Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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About This Item

Code UNSPSC :
41116015
Nomenclature NACRES :
NA.32

Gamme de produits

ColorWheel®

Niveau de qualité

Forme

lyophilized

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

flow cytometry: suitable

Fluorescence

λex 656 nm; λem 670 nm

Compatibilité

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

iFluor 647 succinimidyl ester is bright, stable far-red fluorescent dye that has spectral properties comparable to Alexa Fluor 647 and Cy dye. It exhibits an excitation max at 656 nm and an emission max at 670 nm with an extinction coefficient of 250,000 cm-1 M-1. It has stronger fluorescence and high photostability. iFluor 647 can be used for covalent labeling of antibodies and detection by flow cytometry. Its fluorescence is independent of pH from 3 to 11. It exhibits good stability, selectivity, and reactivity with protein amine groups.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Flow Cytometry: Excitation: 656 nm, Emission: 670 nm

Compatibilité

Requires ColorWheel® Antibody (Sold Separately)

Forme physique

CWDS-IF647-25UL Set Components

CWD-IF647-25UL - Colorwheel® Antibody-Ready iFluor 647 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - Colorwheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Produit(s) apparenté(s)

Code de la classe de stockage

11 - Combustible Solids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Find answers to frequently asked questions (FAQs) about ColorWheel® flow cytometry antibodies and dyes, straight from the experts.

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocoles

Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow cytometry antibodies and dyes.

View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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