CGP1

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

• Synonyme(s) : Cellular glutathione peroxidase, c-GPx
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 200
• Utilisation: sufficient for 100 tests
• Durée de conservation: Kit is stable for 24 months when unopened.
• Méthode de détection: colorimetric
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Adéquation

Suitable for the measureent of glutathione peroxidase in tissue extracts

Principe

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Autres remarques

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Composants de kit seuls

• tert-Butyl hydroperoxide 1 mL

Informations sur la sécurité

• Pictogrammes: GHS02,GHS06,GHS08,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H226,H242,H302,H311,H314,H317,H330,H335,H341,H411
• Conseils de prudence: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P370 + P378
• Classification des risques: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 5.2 - Organic peroxides and self-reacting hazardous materials