8C9

99072805, Reactivity: Anti-CD36, human

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen,female
• Myélome: NS-1
• Réactivité: Anti-CD36, human
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgM
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse x Mouse hybridoma

Description de la lignée cellulaire

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

Immunogène

Human malignant fibrous histiocytoma

Application

Study of endothelial cells, CD36 expression analysis

Milieu de culture

RPMI 1640 + 2mM glutamine + 10% FBS

Procédure de repiquage

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Autres remarques

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