HKT-1097

98061003, hamster kidney, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: HKT-1097, 98061003
• Source biologique: hamster kidney
• Conditionnement: tube of 5 μg 98061003-DNA-5UG; pkg of vial of cells 98061003-1VL
• Mode de croissance: Adherent
• Caryotype: Modal no. 44
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: EGF
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Syrian Hamster kidney

Description de la lignée cellulaire

The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.

Profil d'ADN

Not specified

Milieu de culture

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Form a multipolar, loosely packed monolayer.

Autres remarques

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