3A(tPA-30-1)

96020926, human placenta, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: 3A(tPA-30-1), 96020926
• Source biologique: human placenta
• Mode de croissance: Adherent
• Caryotype: Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.
• Morphologie: Epithelial
• Produits: At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase.
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human placenta, SV40 transformed

Description de la lignée cellulaire

Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1

Milieu de culture

α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 36°C. Change medium 2-3 times weekly.

Autres remarques

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