XrS6-hamKu80

96012301, hamster ovary, Not specified

• Code UNSPSC : 41106514

Propriétés

• product name: XrS6-hamKu80, 96012301
• Source biologique: hamster ovary
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Ku80
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Hamster Chinese ovary, transformed with hamster Ku80 cDNA

Description de la lignée cellulaire

The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.

Application

Study of DNA repair mechanisms

Milieu de culture

Ham′s F12 + 2mM Glutamine + 300μg/ml G418 + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.